Biosynthesis of Shearinine: Diversification of a Tandem Prenyl Moiety of Fungal Indole Diterpenes

Chengwei Liu, Atsushi Minami, Tohru Dairi, Katsuya Gomi, Barry Scott, Hideaki Oikawa

研究成果: Article査読

19 被引用数 (Scopus)

抄録

The late-stage biosynthetic pathway of the indole diterpene shearinine involving four enzymatic reactions (JanQDOJ) was elucidated by an efficient heterologous expression system using Aspergillus oryzae. Key oxidative cyclization, forming a characteristic A/B bicyclic shearinine core by flavoprotein oxidase, was studied using a substrate analogue and a buffer containing H218O. These experimental data provided evidence that JanO catalyzes two-step oxidation via a hydroxylated product and that the JanO reaction involves the hydride-transfer mechanism.

本文言語English
ページ(範囲)5026-5029
ページ数4
ジャーナルOrganic letters
18
19
DOI
出版ステータスPublished - 2016 10 7

ASJC Scopus subject areas

  • 生化学
  • 物理化学および理論化学
  • 有機化学

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