Biomembrane mimetic anti-bioadsorption interface constructed for microfluidic devices

A negatively charged phospholipid polymer PMBSSi oriented to quartz/glass was newly designed and synthesized. Several kinds of proteins, their concentration were 1 % human plasma level, were used to evaluate the coating effect. After PMBSSi coating, the protein adsorptions on all the plates, quartz or glass, were decreased at least several times and the maximum is more than 20 times. Moreover, the absolute amounts of remained proteins on the coating surfaces were at a quite low level, nearly all below 0.5μg/cm². As a conclusion, PMBSSi coating have more potential to be applied in those protein chips whose inner surface not only need anti-adsorption but also need a certain amount of charge to realize or improve special functions such as EOF effect.