Biomechanics of C2C12 Cells Observed with Cellular Resolution Scanning Acoustic Microscope Combined with Optical Microscope

Ryo Hirano, Makoto Kanzaki, Mototaka Arakawa, Norma Hermawan, Kazuto Kobayashi, Yoshifumi Saijo

研究成果: Conference contribution

抄録

Biomechanics of the cell indicates the inner structure and viability of the cell. Mechanical properties are represented by acoustic properties such as speed of sound (SOS) or acoustic impedance. In the present study, cellular resolution scanning acoustic microscope combined with optical microscope (OptSAM) is developed to observe the change of mechanical properties in cell differentiation. Main part of the OptSAM was consisted of 350 MHz ultrasound transducer mechanically scanned by a piezo-actuator. Thickness, SOS, acoustic impedance, density and elastic bulk modulus of the cell were deduced by the ultrasound responses in both time domain and frequency domain. C2C12 cell changing its form from myoblast to myotube was observed by OptSAM. The value of bulk modulus slightly increased in response to differentiation process. OptSAM non-invasively provides important information on biomechanics of cells without contact or staining.

本文言語English
ホスト出版物のタイトル2019 41st Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2019
出版社Institute of Electrical and Electronics Engineers Inc.
ページ4828-4831
ページ数4
ISBN(電子版)9781538613115
DOI
出版ステータスPublished - 2019 7
イベント41st Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2019 - Berlin, Germany
継続期間: 2019 7 232019 7 27

出版物シリーズ

名前Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
ISSN(印刷版)1557-170X

Conference

Conference41st Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2019
国/地域Germany
CityBerlin
Period19/7/2319/7/27

ASJC Scopus subject areas

  • 信号処理
  • 生体医工学
  • コンピュータ ビジョンおよびパターン認識
  • 健康情報学

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