Bioluminescence and magnetic resonance imaging of macrophage homing to experimental abdominal aortic aneurysms

Noriyuki Miyama, Monica M. Dua, Geoffrey M. Schultz, Hisanori Kosuge, Masahiro Terashima, Laura J. Pisani, Ronald L. Dalman, Michael V. McConnell

    研究成果: Article査読

    10 被引用数 (Scopus)

    抄録

    Macrophage infiltration is a prominent feature of abdominal aortic aneurysm (AAA) progression. We used a combined imaging approach with bioluminescence (BLI) and magnetic resonance imaging (MRI) to study macrophage homing and accumulation in experimental AAA disease. Murine AAAs were created via intra-aortic infusion of porcine pancreatic elastase. Mice were imaged over 14 days after injection of prepared peritoneal macrophages. For BLI, macrophages were from transgenic mice expressing luciferase. For MRI, macrophages were labeled with iron oxide particles. Macrophage accumulation during aneurysm progression was observed by in situ BLI and by in vivo 7T MRI. Mice were sacrificed after imaging for histologic analysis. In situ BLI (n = 32) demonstrated high signal in the AAA by days 7 and 14, which correlated significantly with macrophage number and aortic diameter. In vivo 7T MRI (n = 13) at day 14 demonstrated T 2* signal loss in the AAA and not in sham mice. Immunohistochemistry and Prussian blue staining confirmed the presence of injected macrophages in the AAA. BLI and MRI provide complementary approaches to track macrophage homing and accumulation in experimental AAAs. Similar dual imaging strategies may aid the study of AAA biology and the evaluation of novel therapies.

    本文言語English
    ページ(範囲)126-134
    ページ数9
    ジャーナルMolecular Imaging
    11
    2
    DOI
    出版ステータスPublished - 2012 3

    ASJC Scopus subject areas

    • バイオテクノロジー
    • 分子医療
    • 生体医工学
    • 放射線学、核医学およびイメージング
    • 凝縮系物理学

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