Bioelectric responses of sea urchin eggs inseminated with oyster spermatozoa: A sperm evoked potential without egg activation

Kenzi Osanai, Kei ichiro Kyozuka, Hiroshi Sato, Setsuro Hirai, Yukio Igusa, Shun ichi Miyazaki

研究成果: Article査読

3 被引用数 (Scopus)

抄録

Multiple oyster spermatozoa can enter sea urchin eggs with or often without fertilization membrane formation (Osanai and Kyozuka, 1982). In the present work, electrical responses of sea urchin (Temnopleurus hardwicki) eggs inseminated with oyster (Crassostrea gigas) sperm were examined and correlated to the failure of monospermy and egg activation. With diluted sperm, a transient depolarization of the membrane with a constant pattern appeared repeatedly and discretely, and the depolarizations (sperm evoked potentials, SEPs) were not associated with fertilization membrane elevation. With dense sperm, the SEPs occurred consecutively, and sometimes an assembled consecutive depolarization was followed by an activation potential associated with cortical granule discharge. When the membrane potential was artificially held at positive levels, the frequency of SEPs was strongly suppressed but not completely blocked. The present results indicate that an individual heterologous spermatozoon neither produces a depolarization sufficient to block additional sperm entry, nor stimulates egg activation, and that simultaneous entries of multiple heterologous spermatozoa, as possibly reflected by the assembled consecutive depolarizations, induce cortical granule discharge and egg activation.

本文言語English
ページ(範囲)309-315
ページ数7
ジャーナルDevelopmental Biology
124
2
DOI
出版ステータスPublished - 1987 12

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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