Biodegradation of crude oil and phenanthrene by heavy metal resistant Bacillus subtilis isolated from a multi-polluted industrial wastewater creek

Ganiyu Oladunjoye Oyetibo, Mei Fang Chien, Wakako Ikeda-Ohtsubo, Hitoshi Suzuki, Oluwafemi Sunday Obayori, Sunday Adekunle Adebusoye, Matthew Olusoji Ilori, Olukayode Oladipo Amund, Ginro Endo

研究成果: Article査読

32 被引用数 (Scopus)

抄録

A critical bottleneck associated with bioremediation technology in multi-polluted environments is microbiostasis due to metal toxicity. Autochthonous Bacillus species that would harness a repertory of traits to catabolize hydrocarbons and simultaneously sequester heavy metals (HMs) is invaluable in the environment contaminated with divergent pollutants. Fourteen HM-resistant bacilli from polluted creek were characterized using phenotypic and molecular criteria, and studied for hydrocarbon degradation in chemically defined media amended with Co2+ and Ni2+ (5.0 mmol l−1 each). Phylogenetic analyses revealed distribution of the bacilli into three clades. Two dissimilar strains of Bacillus subtilis (M16K, and M19F) with 19.1% sequence divergence, exhibited excellent degradation of crude oil (>94.0%) with evidence of early degradation of isoprenoid hydrocarbons and concurrent metal removal 18 d post-inoculation. Similarly, phenanthrene degradation (>85.0%), and corresponding metal detoxification occurred in 28 d axenic culture of the strains. Strain M16K and M19F were metabolically active in matrices containing HMs, degraded hydrocarbons and simultaneously removed HMs from the medium. To the best of our knowledge, this is the first report of metal-resistant Bacillus subtilis strains showing simultaneous degradation of hydrocarbons and detoxification of metals, particularly in the Sub-Saharan Africa. The bacilli could be useful as potential biological agents in effective bioremediation campaign for multi-polluted environments.

本文言語English
ページ(範囲)143-151
ページ数9
ジャーナルInternational Biodeterioration and Biodegradation
120
DOI
出版ステータスPublished - 2017 5 1

ASJC Scopus subject areas

  • 微生物学
  • 生体材料
  • 廃棄物管理と処理

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