Biodegradable main-chain phosphate-caged fluorescein polymers for the evaluation of enzymatic activity

Kazuo Tanaka, Narufumi Kitamura, Yoshiki Chujo

研究成果: Article査読

16 被引用数 (Scopus)

抄録

We describe here the biodegradable polymers consisted of the tandemly linked phosphate-caged fluorescein in the main chains. The caged-fluorescein monomer with the phosphoramidite groups at both hydroxyl groups was synthesized, and the polycondensations were executed in the presence of the diol derivatives which were promised to be the reactivity-tunable linker. After oxidation and the alkaline treatment for the deprotection, less-emissive polymeric products were obtained. The resulting polymers can release fluorescein by the digestion with alkaline phosphatase. We demonstrated that the enzymatic activity of the cell lysate can be quantified from the increase of the magnitude of fluorescence emission with the synthetic polymers.

本文言語English
ページ(範囲)6180-6184
ページ数5
ジャーナルMacromolecules
43
14
DOI
出版ステータスPublished - 2010 7 27
外部発表はい

ASJC Scopus subject areas

  • 有機化学
  • ポリマーおよびプラスチック
  • 無機化学
  • 材料化学

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