Biochemical sub-fractionation of the mammalian Golgi apparatus

Tomohiko Taguchi, Marc Pypaert, Graham Warren

研究成果: Review article査読

17 被引用数 (Scopus)

抄録

We have exploited the breakdown of the Golgi apparatus that occurs during mitosis to isolate subfractions using immuno-affinity methods. Rat liver Golgi stacks were treated with mitotic cytosol from HeLa cells, and the fragments were then incubated with antibodies immobilized on magnetic beads. Antibodies against the cis-Golgi marker, GM130, bound membranes that were depleted in the trans-Golgi network marker, TGN38, whereas antibodies against the cytoplasmic tail of TGN38 did the reverse. A range of other Golgi enzymes, SNAREs and tethers were also tested and were found to bind to anti-GM130 antibodies to an extent that reflected their proximity to cis-cisternae as determined by other techniques. This method should provide a useful complement to the immuno-EM methods presently used to map the Golgi apparatus.

本文言語English
ページ(範囲)344-352
ページ数9
ジャーナルTraffic
4
5
DOI
出版ステータスPublished - 2003 5月 1
外部発表はい

ASJC Scopus subject areas

  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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