Binding properties of 3H-PbTx-3 and 3H-Saxitoxin to brain membranes and to skeletal muscle membranes of puffer fish Fugu pardalis and the primary structure of a voltage-gated Na+ channel α-subunit (fMNa1) from skeletal muscle of F. pardalis

Mari Yotsu-Yamashita, Katsuhiko Nishimori, Yoko Nitanai, Masako Isemura, Atsuko Sugimoto, Takeshi Yasumoto

研究成果: Article査読

52 被引用数 (Scopus)

抄録

The dissociation constants for 3H-saxitoxin to brain membranes and to skeletal muscle membranes of puffer fish Fugu pardalis have been estimated to be 190- and 460-fold, respectively, larger than those to corresponding membranes of rat, by a rapid filtration assay, while these values for 3H-PbTx-3 have been estimated to be one-third and one-half of those to rat, respectively. We have obtained a cDNA, encoding an entire voltage-gated Na+ channel α-subunit (fMNa1, 1880 residues) from skeletal muscle of F. pardalis by composition of the fragments obtained from cDNA library and RT-PCR products. In fMNa1 protein, the residues for ion-selective filter and voltage sensor and the charged residues in SS2 regions of domains I-IV were conserved, but the aromatic amino acid (Phe/Tyr), commonly located in the SS2 region of domain I of tetrodotoxin-sensitive Na+ channels, was replaced by Asn. With this particular criterion, we propose that the fMNa1 protein is a tetrodotoxin-resistant Na+ channel. (C) 2000 Academic Press.

本文言語English
ページ(範囲)403-412
ページ数10
ジャーナルBiochemical and biophysical research communications
267
1
DOI
出版ステータスPublished - 2000 1 7

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

フィンガープリント

「Binding properties of <sup>3</sup>H-PbTx-3 and <sup>3</sup>H-Saxitoxin to brain membranes and to skeletal muscle membranes of puffer fish Fugu pardalis and the primary structure of a voltage-gated Na<sup>+</sup> channel α-subunit (fMNa1) from skeletal muscle of F. pardalis」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

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