Binding of diarrheic shellfish poisoning toxins to okadaic acid binding proteins purified from the sponge Halichondria okadai

Keiichi Konoki, Kaori Saito, Hiroki Matsuura, Naoyuki Sugiyama, Yuko Cho, Mari Yotsu-Yamashita, Kazuo Tachibana

研究成果: Article査読

10 被引用数 (Scopus)

抄録

Okadaic acid (OA) and dinophysistoxin-1 (DTX1) cause diarrheic shellfish poisoning. This article examines the biochemical interactions of the two toxins with novel okadaic acid binding proteins (OABPs) 2.1 and 2.3, originally isolated from the marine sponge Halichondria okadai. First, recombinant OABPs 2.1 and 2.3 were expressed in Escherichia coli BL21 (DE3) cells. Binding assays using [24-3H]OA and the recombinant OABP 2.1 or 2.3 demonstrated the dissociation constant Kd of 1.30 ± 0.56 nM and 1.54 ± 0.35 nM, respectively. Binding of [24-3H]okadaic acid to recombinant OABP2.1 was almost equally replaced with OA and DTX1. OA-induced cytotoxicity in mouse leukemia P388 cells was inhibited in the presence of the recombinant OABPs 2.1 and 2.3 with an EC50 of 92 ± 8.4 nM and 87 ± 13 nM, respectively. These results suggest that the blockage of OA-induced cytotoxicity by OABPs 2.1 and 2.3 may be involved in regulating symbiotic relationships present in the sponge H. okadai.

本文言語English
ページ(範囲)7607-7610
ページ数4
ジャーナルBioorganic and Medicinal Chemistry
18
21
DOI
出版ステータスPublished - 2010 11 1

ASJC Scopus subject areas

  • 生化学
  • 分子医療
  • 分子生物学
  • 薬科学
  • 創薬
  • 臨床生化学
  • 有機化学

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