BETA2/NeuroD protein can be transduced into cells due to an arginine- and lysine-rich sequence

Hirofomi Noguchi, Susan Bonner-Weir, Fan Yan Wei, Masayuki Matsushita, Shinichi Matsumoto

研究成果: Article査読

97 被引用数 (Scopus)

抄録

BETA2/NeuroD, a basic helix-loop-helix transcription factor, is a key regulator of pancreatic islet morphogenesis and insulin gene transcription. Here we report for the first time that the BETA2/NeuroD protein can permeate several cells, including pancreatic islets, due to an arginine- and lysine-rich protein transduction domain sequence in its structure. The BETA2/NeuroD protein was transduced in a dose-dependent manner up to 1 μmol/l. Transduced BETA2/NeuroD functions similarly to endogenous BETA2/NeuroD: it binds to the insulin promoter and activates its expression. We also investigated the mechanism of BETA2/NeuroD protein transduction. The BETA2/NeuroD protein penetrated cells by macropinocytosis and was released from endosomes homogeneously in cytoplasm and nuclei. These data suggest that BETA2/NeuroD protein transduction could be a safe and valuable strategy for enhancing insulin gene transcription without requiring gene transfer technology.

本文言語English
ページ(範囲)2859-2866
ページ数8
ジャーナルDiabetes
54
10
DOI
出版ステータスPublished - 2005 10
外部発表はい

ASJC Scopus subject areas

  • 内科学
  • 内分泌学、糖尿病および代謝内科学

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