Basolateral sorting of the Mg2+ transporter CNNM4 requires interaction with AP-1A and AP-1B

Yusuke Hirata, Yosuke Funato, Hiroaki Miki

研究成果: Article査読

9 被引用数 (Scopus)

抄録

Ancient conserved domain protein/cyclin M (CNNM) 4 is an evolutionarily conserved Mg2+ transporter that localizes at the basolateral membrane of the intestinal epithelia. Here, we show the complementary importance of clathrin adaptor protein (AP) complexes AP-1A and AP-1B in basolateral sorting of CNNM4. We first confirmed the basolateral localization of both endogenous and ectopically expressed CNNM4 in Madin-Darby Canine Kidney cells, which form highly polarized epithelia in culture. Single knockdown of μ1B, a cargo-recognition subunit of AP-1B, did not affect basolateral localization, but simultaneous knockdown of the μ1A subunit of AP-1A abrogated localization. Mutational analyses showed the importance of three conserved dileucine motifs in CNNM4 for both basolateral sorting and interaction with μ1A and μ1B. These results imply that CNNM4 is sorted to the basolateral membrane by the complementary function of AP-1A and AP-1B.

本文言語English
ページ(範囲)184-189
ページ数6
ジャーナルBiochemical and biophysical research communications
455
3-4
DOI
出版ステータスPublished - 2014 12月 12

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

フィンガープリント

「Basolateral sorting of the Mg2+ transporter CNNM4 requires interaction with AP-1A and AP-1B」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル