Bactericidal Activity and Mechanism of Photoirradiated Polyphenols against Gram-Positive and -Negative Bacteria

Keisuke Nakamura, Kirika Ishiyama, Hong Sheng, Hiroyo Ikai, Taro Kanno, Yoshimi Niwano

研究成果: Article査読

58 被引用数 (Scopus)

抄録

The bactericidal effect of various types of photoirradiated polyphenols against Gram-positive and -negative bacteria was evaluated in relation to the mode of action. Gram-positive bacteria (Enterococcus faecalis, Staphylococcus aureus, and Streptococcus mutans) and Gram-negative bacteria (Aggregatibacter actinomycetemcomitans, Escherichia coli, and Pseudomonas aeruginosa) suspended in a 1 mg/mL polyphenol aqueous solution (caffeic acid, gallic acid, chlorogenic acid, epigallocatechin, epigallocatechin gallate, and proanthocyanidin) were exposed to LED light (wavelength, 400 nm; irradiance, 260 mW/cm2) for 5 or 10 min. Caffeic acid and chlorogenic acid exerted the highest bactericidal activity followed by gallic acid and proanthocyanidin against both Gram-positive and -negative bacteria. It was also demonstrated that the disinfection treatment induced oxidative damage of bacterial DNA, which suggests that polyphenols are incorporated into bacterial cells. The present study suggests that blue light irradiation of polyphenols could be a novel disinfection treatment.

本文言語English
ページ(範囲)7707-7713
ページ数7
ジャーナルJournal of Agricultural and Food Chemistry
63
35
DOI
出版ステータスPublished - 2015 9 9

ASJC Scopus subject areas

  • 化学 (全般)
  • 農業および生物科学(全般)

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