Bach2 represses the AP-1-driven induction of interleukin-2 gene transcription in CD4+ T cells

Eunkyeong Jang, Hye Rim Lee, Geon Hee Lee, Ah Reum Oh, Ji Young Cha, Kazuhiko Igarashi, Jeehee Youn

研究成果: Article査読

7 被引用数 (Scopus)

抄録

The transcription repressor Bach2 has been proposed as a regulator of T cell quiescence, but the underlying mechanism is not fully understood. Given the importance of interleukin-2 in T cell activation, we investigated whether Bach2 is a component of the network of factors that regulates interleukin-2 expression. In primary and transformed CD4+ T cells, Bach2 overexpression counteracted T cell receptor/CD28- or PMA/ ionomycin-driven induction of interleukin-2 expression, and silencing of Bach2 had the opposite effect. Luciferase and chromatin immunoprecipitation assays revealed that Bach2 binds to multiple Maf-recognition element-like sites on the interleukin-2 proximal promoter in a manner competitive with AP-1, and thereby represses AP-1-driven induction of interleukin-2 transcription. Thus, this study demonstrates that Bach2 is a direct repressor of the interleukin-2 gene in CD4+ T cells during the immediate early phase of AP-driven activation, thereby playing an important role in the maintenance of immune quiescence in the steady state.

本文言語English
ページ(範囲)472-477
ページ数6
ジャーナルBMB Reports
50
9
DOI
出版ステータスPublished - 2017

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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