Atomic force microscopic imaging of the intracellular membrane surface of prestin-expressing Chinese hamster ovary cells

H. Wada, M. Murakoshi, K. Iida, S. Kumano, T. Gomi, K. Kimura, H. Usukura, M. Sugawara, S. Kakehata, K. Ikeda, Y. Katori, T. Kobayashi

研究成果: Conference contribution

抜粋

The high sensitivity of human hearing is believed to be achieved by cochlear amplification. The basis of this amplification is thought to be the motility of mammalian outer hair cells (OHCs), i.e., OHCs elongate and contract in response to acoustical stimulation. This motility is made possible by both the cytoskeleton beneath the OHC plasma membrane and the motor protein prestin distributed throughout the plasma membrane. However, these factors have not yet been fully clarified. In the present study, therefore, attempts were made to observe the ultrastructure of the cytoskeleton of Guinea pig OHCs and to identify the motor protein prestin expressed in the plasma membrane of Chinese hamster ovary (CHO) cells by atomic force microscopy (AFM). Results indicate that the OHC cytoskeleton is comprised of circumferential actin filaments and spectrin cross-links and that particle-like structures with a diameter of 8–12 nm which exist in the plasma membrane of the prestin-expressing CHO cells are most likely be prestin.

元の言語English
ホスト出版物のタイトルAuditory Mechanisms
ホスト出版物のサブタイトルProcesses and Models - Proceedings of the 9th International Symposium
編集者Alfred L. Nuttall, Tianying Ren, Peter Gillespie, Karl Grosh, Egbert de Boer
出版者World Scientific Publishing Co. Pte Ltd
ページ26-33
ページ数8
ISBN(電子版)9812568247, 9789812568243
出版物ステータスPublished - 2005
イベント9th International Mechanics of Hearing Workshop on Auditory Mechanisms: Processes and Models, MoH 2005 - Portland, United States
継続期間: 2005 7 232005 7 28

出版物シリーズ

名前Auditory Mechanisms: Processes and Models - Proceedings of the 9th International Symposium

Conference

Conference9th International Mechanics of Hearing Workshop on Auditory Mechanisms: Processes and Models, MoH 2005
United States
Portland
期間05/7/2305/7/28

ASJC Scopus subject areas

  • Biotechnology
  • Biomedical Engineering

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  • これを引用

    Wada, H., Murakoshi, M., Iida, K., Kumano, S., Gomi, T., Kimura, K., Usukura, H., Sugawara, M., Kakehata, S., Ikeda, K., Katori, Y., & Kobayashi, T. (2005). Atomic force microscopic imaging of the intracellular membrane surface of prestin-expressing Chinese hamster ovary cells. : A. L. Nuttall, T. Ren, P. Gillespie, K. Grosh, & E. de Boer (版), Auditory Mechanisms: Processes and Models - Proceedings of the 9th International Symposium (pp. 26-33). (Auditory Mechanisms: Processes and Models - Proceedings of the 9th International Symposium). World Scientific Publishing Co. Pte Ltd.