Aryl hydrocarbon receptor/dioxin receptor in human monocytes and macrophages

K. Komura, S. I. Hayashi, I. Makino, L. Poellinger, H. Tanaka

研究成果: Article査読

32 被引用数 (Scopus)

抄録

Aryl hydrocarbon receptor (AhR) belongs to the bHLH/PAS transcription factor family and is activated by various polycyclic or halogenated aromatic hydrocarbons, e.g. 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), 3-methylcholanthrene (3MC). In the present study, we showed that in U937 cells and human macrophages AhR, with its partner cofactor Arnt, is expressed and CYP1A1 mRNA expression is induced in the presence of AhR ligand 3MC. Moreover, we showed that AhR, associating with Arnt, binds to target DNA sequences and activates transcription. Since part of AhR is activated into DNA binding species in the absence of exogenous ligand and competitive AhR antagonist α-naphthoflavone inhibits this activation process with reducing CYP1A1 mRNA expression levels, the presence of endogenous ligand is indicated.

本文言語English
ページ(範囲)107-117
ページ数11
ジャーナルMolecular and Cellular Biochemistry
226
1-2
DOI
出版ステータスPublished - 2001
外部発表はい

ASJC Scopus subject areas

  • 分子生物学
  • 臨床生化学
  • 細胞生物学

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