Arid5b facilitates chondrogenesis by recruiting the histone demethylase Phf2 to Sox9-regulated genes

Kenji Hata, Rikako Takashima, Katsuhiko Amano, Koichiro Ono, Masako Nakanishi, Michiko Yoshida, Makoto Wakabayashi, Akio Matsuda, Yoshinobu Maeda, Yutaka Suzuki, Sumio Sugano, Robert H. Whitson, Riko Nishimura, Toshiyuki Yoneda

研究成果: Article

41 引用 (Scopus)

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Histone modification, a critical step for epigenetic regulation, is an important modulator of biological events. Sox9 is a transcription factor critical for endochondral ossification; however, proof of its epigenetic regulation remains elusive. Here we identify AT-rich interactive domain 5b (Arid5b) as a transcriptional co-regulator of Sox9. Arid5b physically associates with Sox9 and synergistically induces chondrogenesis. Growth of Arid5b-/- mice is retarded with delayed endochondral ossification. Sox9-dependent chondrogenesis is attenuated in Arid5b-deficient cells. Arid5b recruits Phf2, a histone lysine demethylase, to the promoter region of Sox9 target genes and stimulates H3K9me2 demethylation of these genes. In the promoters of chondrogenic marker genes, H3K9me2 levels are increased in Arid5b-/- chondrocytes. Finally, we show that Phf2 knockdown inhibits Sox9-induced chondrocyte differentiation. Our findings establish an epigenomic mechanism of skeletal development, whereby Arid5b promotes chondrogenesis by facilitating Phf2-mediated histone demethylation of Sox9-regulated chondrogenic gene promoters.

元の言語English
記事番号2850
ジャーナルNature communications
4
DOI
出版物ステータスPublished - 2013 11 26
外部発表Yes

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

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    Hata, K., Takashima, R., Amano, K., Ono, K., Nakanishi, M., Yoshida, M., Wakabayashi, M., Matsuda, A., Maeda, Y., Suzuki, Y., Sugano, S., Whitson, R. H., Nishimura, R., & Yoneda, T. (2013). Arid5b facilitates chondrogenesis by recruiting the histone demethylase Phf2 to Sox9-regulated genes. Nature communications, 4, [2850]. https://doi.org/10.1038/ncomms3850