Application of planar chromatography to the determination of continine in urine of active and passive smoking pregnant women

Toshimasa Toyo'oka, Dongri Jin, Nobue Tomoi, Tomoyuki Oe, Hidenori Hiranuma

研究成果: Article査読

15 被引用数 (Scopus)

抄録

A quick and economical method for the determination of nicotine and its main metabolite-cotinine in urine from smoking and passively smoking pregnant women is proposed using TLC-densitometry. Chromatographic separations were performed on the plates coated with silica gel bound with C-18 alkyl chains. Chromatograms were developed using a mixture of acetonitrile and water (88 + 12, v/v) in a horizontal chamber. Visualization was carried out under UV illumination at λ = 254 nm and then cotinine was quantified by the use of densitometer in reflectance mode at λ = 260 nm. Nicotine was identified after derivatization with Dragendorff reagent under visible light. The results of nicotine and cotinine analysis were verified by questionnaires obtained from pregnant women according to their age and smoking status correlated with spontaneous abortion frequency and child death rate.

本文言語English
ページ(範囲)50-55
ページ数6
ジャーナルBiomedical Chromatography
15
1
DOI
出版ステータスPublished - 2001 3 3
外部発表はい

ASJC Scopus subject areas

  • 分析化学
  • 生化学
  • 分子生物学
  • 薬理学
  • 創薬
  • 臨床生化学

フィンガープリント

「Application of planar chromatography to the determination of continine in urine of active and passive smoking pregnant women」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル