Application of nanosecond pulsed electric fields into HeLa cells expressing enhanced green fluorescent protein and fluorescence lifetime microscopy

Kamlesh Awasthi, Takakazu Nakabayashi, Nobuhiro Ohta

研究成果: Article査読

11 被引用数 (Scopus)

抄録

An electrode microchamber has been constructed for applying nanosecond pulsed strong electric fields to living cells, and fluorescence lifetime microscopy (FLIM) has been used to investigate the effects of external electric fields on dynamics and function of HeLa cells expressing enhanced green fluorescent protein (EGFP). Both morphological change in cells and reduction of the fluorescence lifetime of EGFP have been observed after application of electric fields having a pulsed width of 50 ns and a strength of 4 MV m -1, indicating that apoptosis, which is a programmed cell death, was induced by nanosecond pulsed electric fields and that fluorescence lifetime of EGFP decreased along with the induction of apoptosis. The reduction of the fluorescence lifetime occurred before the morphological change, indicating that FLIM provides a sensitive and noninvasive detection of the progress of apoptosis induced by application of nanosecond pulsed electric fields.

本文言語English
ページ(範囲)11159-11165
ページ数7
ジャーナルJournal of Physical Chemistry B
116
36
DOI
出版ステータスPublished - 2012 9 13
外部発表はい

ASJC Scopus subject areas

  • 物理化学および理論化学
  • 表面、皮膜および薄膜
  • 材料化学

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