Application of modular kinetic analysis to mitochondrial oxidative phosphorylation in skeletal muscle of birds exposed to acute heat stress

Motoi Kikusato, Jon J. Ramsey, Taku Amo, Masaaki Toyomizu

研究成果: Article査読

20 被引用数 (Scopus)

抄録

We previously showed that heat stress stimulates reactive oxygen species (ROS) production in skeletal muscle mitochondria of birds, probably via an elevation in mitochondrial membrane potential (ΔΨ). To clarify the mechanism underlying the elevation of ΔΨ, modular kinetic analysis was applied to oxidative phosphorylation in skeletal muscle mitochondria of heat-stressed birds (34°C for 12. h). In the birds exposed to heat stress, 'substrate oxidation' (a ΔΨ-producer) was increased compared to control (24°C) birds, although there was little difference in 'proton leak' (a ΔΨ-consumer), suggesting that an elevation in the ΔΨ at state 4 may be due to enhanced substrate oxidation. It thus appears that enhanced substrate oxidation plays a crucial role in the overproduction of ROS for heat-stressed birds, probably via elevated ΔΨ.

本文言語English
ページ(範囲)3143-3148
ページ数6
ジャーナルFEBS Letters
584
14
DOI
出版ステータスPublished - 2010 7

ASJC Scopus subject areas

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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