Application of fluorescence lifetime imaging (FLIM) to measure intracellular environments in a single cell

Takakazu Nakabayashi, Kamlesh Awasthi, Nobuhiro Ohta

研究成果: Chapter

抄録

Fluorescence lifetime imaging (FLIM) has now been used in many bioscience fields, which comes from the quantification of fluorescence lifetime. The procedure for obtaining lifetime images is very similar to that used in fluorescence microscopy. However, obtaining reliable lifetime images requires an understanding of the theory of fluorescence lifetime, principle of FLIM systems, and evaluation procedure of intracellular environments. In this chapter, the materials, methods, and notes on FLIM measurements have been described, in conjunction with a brief explanation of the background of FLIM.

本文言語English
ホスト出版物のタイトルAdvances in Experimental Medicine and Biology
出版社Springer New York LLC
ページ121-133
ページ数13
DOI
出版ステータスPublished - 2017 1 1

出版物シリーズ

名前Advances in Experimental Medicine and Biology
1035
ISSN(印刷版)0065-2598
ISSN(電子版)2214-8019

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)

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