Application of an enzymatic cascade reaction for the synthesis of the emeraldine salt form of polyaniline

Minoru Kurisu, Reinhard Kissner, Masayuki Imai, Peter Walde

研究成果: Article査読

1 被引用数 (Scopus)

抄録

The synthesis of the emeraldine salt form of polyaniline (PANI-ES) from aniline with Aspergillus sp. glucose oxidase (GOD), d-glucose, dissolved O2, and horseradish peroxidase isoenzyme C (HRPC) in the presence of large unilamellar vesicles of AOT (sodium bis-(2-ethylhexyl)sulfosuccinate) as templates at pH = 4.3 and T ~ 25 °C was investigated in a systematic way. In this cascade reaction mixture, the oxidation of aniline is catalyzed by HRPC with H2O2 that is formed in situ as byproduct of the GOD-catalyzed oxidation of d-glucose with O2. Under the elaborated experimental conditions which we considered ideal, the formation of PANI-ES products is evident, as judged by UV/Vis/NIR and EPR measurements. Comparison was made with a reference reaction, which was run under similar conditions with added H2O2 instead of GOD and d-glucose. Although the reference reaction was found to be superior, with the cascade reaction, PANI-ES products can still be obtained with high aniline conversion (> 90%) within 24 h as stable dark green PANI-ES/AOT vesicle dispersion. Our results show that the in situ formation of H2O2 does not prevent the inactivation of HRPC known to occur in the reference reaction. Moreover, the GOD used in the cascade reaction is inactivated as well by polymerization intermediates.

本文言語English
ページ(範囲)5071-5085
ページ数15
ジャーナルChemical Papers
75
10
DOI
出版ステータスPublished - 2021 10

ASJC Scopus subject areas

  • 生化学
  • 化学 (全般)
  • 化学工学(全般)
  • 産業および生産工学
  • 材料化学

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