Apple latent spherical virus vectors for reliable and effective virus-induced gene silencing among a broad range of plants including tobacco, tomato, Arabidopsis thaliana, cucurbits, and legumes

Aki Igarashi, Kousuke Yamagata, Tomokazu Sugai, Yukari Takahashi, Emiko Sugawara, Akihiro Tamura, Hajime Yaegashi, Noriko Yamagishi, Tsubasa Takahashi, Masamichi Isogai, Hideki Takahashi, Nobuyuki Yoshikawa

研究成果: Article査読

140 被引用数 (Scopus)

抄録

Apple latent spherical virus (ALSV) vectors were evaluated for virus-induced gene silencing (VIGS) of endogenous genes among a broad range of plant species. ALSV vectors carrying partial sequences of a subunit of magnesium chelatase (SU) and phytoene desaturase (PDS) genes induced highly uniform knockout phenotypes typical of SU and PDS inhibition on model plants such as tobacco and Arabidopsis thaliana, and economically important crops such as tomato, legume, and cucurbit species. The silencing phenotypes persisted throughout plant growth in these plants. In addition, ALSV vectors could be successfully used to silence a meristem gene, proliferating cell nuclear antigen and disease resistant N gene in tobacco and RCY1 gene in A. thaliana. As ALSV infects most host plants symptomlessly and effectively induces stable VIGS for long periods, the ALSV vector is a valuable tool to determine the functions of interested genes among a broad range of plant species.

本文言語English
ページ(範囲)407-416
ページ数10
ジャーナルVirology
386
2
DOI
出版ステータスPublished - 2009 4 10

ASJC Scopus subject areas

  • ウイルス学

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