Apoptotic effects on cultured cells of atmospheric-pressure plasma produced using various gases

研究成果: Article査読

1 被引用数 (Scopus)

抄録

This study investigated the effects of low-temperature atmospheric-pressure plasma on various cells such as rat fibroblastic Rat-1 cell line, rat neuroblastoma-like PC12 cell line, and rat macrophage-like NR8383 cell line. The plasma was irradiated directly to a culture medium containing plated cells for 0-20 s. The applied voltage, excitation frequency, and argon or helium gas flow were, respectively, 3-6 kV, 10 kHz, and 3 L/min. Cell viability and apoptotic activity were evaluated using annexin-V/propidium iodide staining. Results showed that the low-temperature atmospheric-pressure plasma irradiation promoted cell death in a discharge-voltage-dependent and irradiation-time-dependent manner. Furthermore, different effects are produced depending on the cell type. Moreover, entirely different mechanisms might be responsible for the induction of apoptosis in cells by helium and argon plasma.

本文言語English
論文番号01AF03
ジャーナルJapanese journal of applied physics
55
1
DOI
出版ステータスPublished - 2016 1月

ASJC Scopus subject areas

  • 工学(全般)
  • 物理学および天文学(全般)

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