Apoptosis occurs in granulosa cells but not cumulus cells in the atretic graafian follicles in multiparous pig ovaries

Noboru Manabe, Yuzuru Imai, Akira Myoumoto, Yoshihiro Kimura, Miki Sugimoto, Yoshinori Okamura, Manabu Fukumoto, Kazuhiro Sakamaki, Hajime Miyamoto

研究成果: Article査読

22 被引用数 (Scopus)

抄録

The process of apoptosis in granulosa and cumulus cells of ovarian follicles during follicular atresia was investigated in multiparous pigs. In healthy follicles, no apoptotic cells were observed histochemically, and no DNA ladder formation or Ca2+/Mg2+-dependent endonuclease activity were detected biochemically in granulosa or cumulus cells. In the follicles in the early stage of atresia, apoptosis occurred in granulosa cells located on the inner surface of the follicular wall but not cumulus cells. DNA ladder formation was observed in granulosa but not cumulus cells, and high levels of endonuclease activity were demonstrated only in the granulosa cells. No endonuclease activity was detected in cumulus cells obtained from the same atretic follicles. These findings suggest a lack of apoptotic cell death in cumulus cells in atretic follicles in multiparous pig ovaries.

本文言語English
ページ(範囲)85-92
ページ数8
ジャーナルACTA HISTOCHEMICA ET CYTOCHEMICA
30
1
DOI
出版ステータスPublished - 1997

ASJC Scopus subject areas

  • 病理学および法医学
  • 生化学
  • 生理学
  • 組織学
  • 細胞生物学

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