Apoptosis by RGD-containing peptides observed in hemocytes of the Pacific oyster, Crassostrea gigas

Kazutaka Terahara, Keisuke G. Takahashi, Katsuyoshi Mori

研究成果: Article査読

27 被引用数 (Scopus)

抄録

We observed in vitro that after treatment with the Arg-Gly-Asp (RGD) peptide, non-spreading Crassostrea gigas hemocytes underwent cell death. Utilizing a combination of a Hoechst staining method and a DNA fragmentation assay, the typical features of apoptosis were shown, i.e. cell shrinkage, membrane blebbing, chromatin condensation, and DNA fragmentation. The hemocyte cell death caused by the RGD peptide appears to be sequence-specific, since no induction was shown in the alanine-substituted control peptide (RAD) treatment. Interestingly, the glutamic acid-substituted control peptide (RGE) also induced hemocytic cell death, but a different type of the death to that induced by the RGD peptide. This is the first report that specific peptides induce cell death in molluscan hemocytes.

本文言語English
ページ(範囲)521-528
ページ数8
ジャーナルDevelopmental and Comparative Immunology
27
6-7
DOI
出版ステータスPublished - 2003 6

ASJC Scopus subject areas

  • 免疫学
  • 発生生物学

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