Antimelanogenesis effect of Tunisian herb Thymelaea hirsuta extract on B16 murine melanoma cells

Mitsuko Kawano, Kyoko Matsuyama, Yusaku Miyamae, Hiroshi Shinmoto, Mohamed Elyes Kchouk, Takahiro Morio, Hideyuki Shigemori, Hiroko Isoda

研究成果: Article査読

48 被引用数 (Scopus)

抄録

Skin pigmentation is the result of melanogenesis that occurs in melanocytes and/or melanoma cells. Although melanogenesis is necessary for the prevention of DNA damage and cancer caused by UV irradiation, excessive accumulation of melanin can also cause melanoma. Thus, we focused on the antimelanogenesis effect of an extract from Thymelaea hirsuta, a Tunisian herb. Murine melanoma B16 cells were treated with T. hirsuta extract, and then cell viability and synthesized melanin content were measured. We found that the T. hirsuta extract decreased the synthesized melanin content in B16 cells without cytotoxicity. Tyrosinase is a key enzyme of melanogenesis and extracellular signal-regulated kinase (ERK)-1/2 phosphorylation is known to be related to melanogenesis inhibition. To clarify its mechanism, we also determined ERK1/2 phosphorylation and tyrosinase expression level. ERK1/2 was immediately phosphorylated in cells just after treatment with the extract. The tyrosinase expression was inhibited after 24 h of stimulation with the extract. The T. hirsuta extract was fractionated, and we found that one fraction considerably decreased the melanin synthesis in B16 cells and that this fraction contains daphnanes as the main component. This indicates that our findings might be attributable to daphnanes.

本文言語English
ページ(範囲)977-984
ページ数8
ジャーナルExperimental Dermatology
16
12
DOI
出版ステータスPublished - 2007 12 1

ASJC Scopus subject areas

  • 生化学
  • 分子生物学
  • 皮膚病学

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