Antibiotic diffusion pathways in the outer membrane of Pseudomonas aeruginosa

Takashi Kitahara, Hiroshi Yoneyama, Taiji Nakae

研究成果: Article査読

6 被引用数 (Scopus)

抄録

We investigated the effect of a temperature shift from 37°C to 17°C on the steady-state diffusion rate of imipenem and cephalothin by evaluating periplasmic drug concentrations in intact cells of Pseudomonas aeruginosa, which overexpresses the extended spectrum β-lactamase. We found that the ratio of periplasmic imipenem concentration at 17°C relative to that of 37°C was 1.03 ± 0.1, whereas that of cephalothin was 0.43 ± 0.09. Accumulation rates of cell-associated tetracycline and fluoroquinolone at 17°C were roughly 1/16 and 1/8, respectively, compared with that at 37°C. We concluded from these data that cephalothin and possibly most other antibiotics excepting carbapenems cross the outer membrane of P. aeruginosa mainly by dissolving in the lipid phase but probably not passing through the porin channel. This may explain why the outer membrane of P. aeruginosa is a tight barrier against the penetration of antibiotics.

本文言語English
ページ(範囲)457-461
ページ数5
ジャーナルBiochemical and biophysical research communications
238
2
DOI
出版ステータスPublished - 1997 9 18
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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