Anti-lamprey retinal antibodies: immunohistochemistry on the retinas of several species of vertebrates

Mika Yoshida, Susumu Saito, Yoshio Koike, Makoto Ishikawa, Hiroshi Watanabe, Fumio Tokunaga, Akira Tonosaki

研究成果: Article査読

7 被引用数 (Scopus)

抄録

Two monoclonal antibodies, H16 and B11, which were raised against lamprey retinal homogenate, were found to react with both short and long photoreceptor outer segments. On Western blotting of the retinal homogenate, both antibodies recognized a 40000 Da and a 80000 Da band. H16 antibody stained rod outer segments of all examined vertebrates, all cone outer segments of the turtle and chicken, and certain cone outer segments of the macaque. B11 antibody stained sub-mammalian rod outer segments and some mammalian cone outer segments, leaving all mammalian rod outer segments unstained. The epitope recognized by H16 antibody is considered to be located in a conserved or commonly inherited element of an outer segment-bound molecule, presumably rhodopsin. B11 antibody, on the other hand, seems to recognize a reactive group which has failed to be inherited by mammalian rod cells; why it recognizes all cone outer segments in the turtle and chicken and only a part of them in the cow, cat, and macaque, meanwhile ignoring all of them in the frog and fish, is subject to further study.

本文言語English
ページ(範囲)419-426
ページ数8
ジャーナルCell and Tissue Research
266
3
DOI
出版ステータスPublished - 1991 12
外部発表はい

ASJC Scopus subject areas

  • 病理学および法医学
  • 組織学
  • 細胞生物学

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