Anterograde Transport of TrkB in Axons Is Mediated by Direct Interaction with Slp1 and Rab27

Nariko Arimura, Toshihide Kimura, Shinichi Nakamuta, Shinichiro Taya, Yasuhiro Funahashi, Atsushi Hattori, Akiko Shimada, Céline Ménager, Saeko Kawabata, Kayo Fujii, Akihiro Iwamatsu, Rosalind A. Segal, Mitsunori Fukuda, Kozo Kaibuchi

研究成果: Article

128 引用 (Scopus)

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The neurotrophin receptors TrkA, TrkB, and TrkC are localized at the surface of the axon terminus and transmit key signals from brain-derived neurotrophic factor (BDNF) for diverse effects on neuronal survival, differentiation, and axon formation. Trk receptors are sorted into axons via the anterograde transport of vesicles and are then inserted into axonal plasma membranes. However, the transport mechanism remains largely unknown. Here, we show that the Slp1/Rab27B/CRMP-2 complex directly links TrkB to Kinesin-1, and that this association is required for the anterograde transport of TrkB-containing vesicles. The cytoplasmic tail of TrkB binds to Slp1 in a Rab27B-dependent manner, and CRMP-2 connects Slp1 to Kinesin-1. Knockdown of these molecules by siRNA reduces the anterograde transport and membrane targeting of TrkB, thereby inhibiting BDNF-induced ERK1/2 phosphorylation in axons. Our data reveal a molecular mechanism for the selective anterograde transport of TrkB in axons and show how the transport is coupled to BDNF signaling.

元の言語English
ページ(範囲)675-686
ページ数12
ジャーナルDevelopmental cell
16
発行部数5
DOI
出版物ステータスPublished - 2009 5 19

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Developmental Biology
  • Cell Biology

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    Arimura, N., Kimura, T., Nakamuta, S., Taya, S., Funahashi, Y., Hattori, A., Shimada, A., Ménager, C., Kawabata, S., Fujii, K., Iwamatsu, A., Segal, R. A., Fukuda, M., & Kaibuchi, K. (2009). Anterograde Transport of TrkB in Axons Is Mediated by Direct Interaction with Slp1 and Rab27. Developmental cell, 16(5), 675-686. https://doi.org/10.1016/j.devcel.2009.03.005