Analysis of the self-assembly process of Aspergillus oryzae hydrophobin RolA by Langmuir–Blodgett method

Yuki Terauchi, Takumi Tanaka, Masaya Mitsuishi, Hiroshi Yabu, Akira Yoshimi, Kei Nantani, Keietsu Abe

研究成果: Article査読

抄録

Hydrophobins are small, amphipathic proteins secreted by filamentous fungi. Hydrophobin RolA, which is produced by Aspergillus oryzae, attaches to solid surfaces, recruits the polyesterase CutL1, and consequently promotes hydrolysis of polyesters. Because this interaction requires the N-terminal, positively charged residue of RolA to be exposed on the solid surface, the orientation of RolA on the solid surface is important for recruitment. However, the process by which RolA forms the self-assembled structure at the interface remains unclear. Using the Langmuir–Blodgett technique, we analyzed the process by which RolA forms a self-assembled structure at the air–water interface and observed the structures on the hydrophobic or hydrophilic SiO2 substrates via atomic force microscopy. We found that RolA formed self-assembled films in two steps during phase transitions. We observed different assembled structures of RolA on hydrophilic and hydrophobic SiO2 substrates.

本文言語English
ページ(範囲)678-685
ページ数8
ジャーナルBioscience, Biotechnology and Biochemistry
84
4
DOI
出版ステータスPublished - 2020 4 2

ASJC Scopus subject areas

  • バイオテクノロジー
  • 分析化学
  • 生化学
  • 応用微生物学とバイオテクノロジー
  • 分子生物学
  • 有機化学

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