TY - JOUR
T1 - Analysis of the complete plastid genome of the unicellular red alga Porphyridium purpureum
AU - Tajima, Naoyuki
AU - Sato, Shusei
AU - Maruyama, Fumito
AU - Kurokawa, Ken
AU - Ohta, Hiroyuki
AU - Tabata, Satoshi
AU - Sekine, Kohsuke
AU - Moriyama, Takashi
AU - Sato, Naoki
N1 - Funding Information:
Acknowledgments The present study was supported in part by a grant for the Global COE Program, ‘‘From the Earth to ‘‘Earths’’’’, from the Ministry of Education, Culture, Sports, Science and Technology of Japan, and by a CREST grant from the Japan Science and Technology Agency.
PY - 2014/5
Y1 - 2014/5
N2 - We determined the complete nucleotide sequence of the plastid genome of the unicellular marine red alga Porphyridium purpureum strain NIES 2140, belonging to the unsequenced class Porphyridiophyceae. The genome is a circular DNA composed of 217,694 bp with the GC content of 30.3 %. Twenty-nine of the 224 protein-coding genes contain one or multiple intron(s). A group I intron was found in the rpl28 gene, whereas the other introns were group II introns. The P. purpureum plastid genome has one non-coding RNA (ncRNA) gene, 29 tRNA genes and two nonidentical ribosomal RNA operons. One rRNA operon has a tRNAAla(UGC) gene between the rrs and the rrl genes, whereas another has a tRNAIle(GAU) gene. Phylogenetic analyses suggest that the plastids of Heterokontophyta, Cryptophyta and Haptophyta originated from the subphylum Rhodophytina. The order of the genes in the ribosomal protein cluster of the P. purpureum plastid genome differs from that of other Rhodophyta and Chromalveolata. These results suggest that a large-scale rearrangement occurred in the plastid genome of P. purpureum after its separation from other Rhodophyta.
AB - We determined the complete nucleotide sequence of the plastid genome of the unicellular marine red alga Porphyridium purpureum strain NIES 2140, belonging to the unsequenced class Porphyridiophyceae. The genome is a circular DNA composed of 217,694 bp with the GC content of 30.3 %. Twenty-nine of the 224 protein-coding genes contain one or multiple intron(s). A group I intron was found in the rpl28 gene, whereas the other introns were group II introns. The P. purpureum plastid genome has one non-coding RNA (ncRNA) gene, 29 tRNA genes and two nonidentical ribosomal RNA operons. One rRNA operon has a tRNAAla(UGC) gene between the rrs and the rrl genes, whereas another has a tRNAIle(GAU) gene. Phylogenetic analyses suggest that the plastids of Heterokontophyta, Cryptophyta and Haptophyta originated from the subphylum Rhodophytina. The order of the genes in the ribosomal protein cluster of the P. purpureum plastid genome differs from that of other Rhodophyta and Chromalveolata. These results suggest that a large-scale rearrangement occurred in the plastid genome of P. purpureum after its separation from other Rhodophyta.
KW - Genome rearrangement
KW - Plastid genome
KW - Porphyridium purpureum
KW - Rhodophyta
KW - rRNA operon
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U2 - 10.1007/s10265-014-0627-1
DO - 10.1007/s10265-014-0627-1
M3 - Article
C2 - 24595640
AN - SCOPUS:84899979576
SN - 0918-9440
VL - 127
SP - 389
EP - 397
JO - Journal of Plant Research
JF - Journal of Plant Research
IS - 3
ER -
