Analysis of the chemotactic behaviour of Leptospira using microscopic agar-drop assay

Md Shafiqul Islam, Kyosuke Takabe, Seishi Kudo, Shuichi Nakamura

研究成果: Article査読

13 被引用数 (Scopus)

抄録

Chemotaxis allows bacterial cells to migrate towards or away from chemical compounds. In the present study, we developed a microscopic agar-drop assay (MAA) to investigate the chemotactic behaviour of a coiled spirochete, Leptospira biflexa. An agar drop containing a putative attractant or repellent was placed around the centre of a flow chamber and the behaviour of free-swimming cells was analysed under a microscope. MAA showed that L. biflexa cells gradually accumulated around an agar drop that contained an attractant such as glucose. Leptospira cells often spin without migration by transformation of their cell body. The frequency at which cells showed no net displacement decreased with a higher glucose concentration, suggesting that sensing an attractive chemical allows these cells to swim more smoothly. Investigation of the chemotactic behaviour of these cells in response to different types of sugars showed that fructose and mannitol induced negative chemotactic responses, whereas xylose and lactose were non-chemotactic for L. biflexa. The MAA developed in this study can be used to investigate other chemoattractants and repellents.

本文言語English
ページ(範囲)39-44
ページ数6
ジャーナルFEMS Microbiology Letters
356
1
DOI
出版ステータスPublished - 2014 7

ASJC Scopus subject areas

  • 微生物学
  • 分子生物学
  • 遺伝学

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