Analysis of target sequences of DDM1s in Brassica rapa by MSAP

Taku Sasaki, Ryo Fujimoto, Sachie Kishitani, Takeshi Nishio

研究成果: Article査読

16 被引用数 (Scopus)

抄録

DNA methylation is an important epigenetic modification regulating gene expression and transposon silencing. Although epigenetic regulation is involved in some agricultural traits, there has been relatively little research on epigenetic modifications of genes in Brassica rapa, which includes many important vegetables. In B. rapa, orthologs of DDM1, a chromatin remodeling factor required for maintenance of DNA methylation, have been characterized and DNA hypomethylated knock-down plants by RNAi (ddm1-RNAi plants) have been generated. In this study, we investigated differences of DNA methylation status at the genome-wide level between a wild-type (WT) plant and a ddm1-RNAi plant by methylation-sensitive amplification polymorphism (MSAP) analysis. MSAP analysis detected changes of DNA methylation of many repetitive sequences in the ddm1-RNAi plant. Search for body methylated regions in the WT plant revealed no difference in gene body methylation levels between the WT plant and the ddm1-RNAi plant. These results indicate that repetitive sequences are preferentially methylated by DDM1 genes in B. rapa.

本文言語English
ページ(範囲)81-88
ページ数8
ジャーナルPlant Cell Reports
30
1
DOI
出版ステータスPublished - 2011 1

ASJC Scopus subject areas

  • 農業および作物学
  • 植物科学

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