TY - JOUR
T1 - Analysis of Sugar Compositions of Glycoproteins Electroblotted onto PVDF Membranes
AU - Muramoto, Koji
AU - Kamiya, Hisao
PY - 1991/1/1
Y1 - 1991/1/1
N2 - A method was developed for analysis of sugar compositions of glycoproteins electroblotted onto polyvinylidene difluoride (PVDF) membranes after SDS-polyacrylamide gel electrophoresis. It was based on hydrolysis of glycoproteins on PVDF membranes and precolumn derivatization of liberated neutral sugars, sialic acids and aminosugars, respectively, and isocratic reversed-phase high-performance liquid chromatographies of these derivatives. The presence of sialoglycoproteins in ecbinoderm shell matrixes was demonstrated by this method.
AB - A method was developed for analysis of sugar compositions of glycoproteins electroblotted onto polyvinylidene difluoride (PVDF) membranes after SDS-polyacrylamide gel electrophoresis. It was based on hydrolysis of glycoproteins on PVDF membranes and precolumn derivatization of liberated neutral sugars, sialic acids and aminosugars, respectively, and isocratic reversed-phase high-performance liquid chromatographies of these derivatives. The presence of sialoglycoproteins in ecbinoderm shell matrixes was demonstrated by this method.
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U2 - 10.2331/suisan.57.1121
DO - 10.2331/suisan.57.1121
M3 - Article
AN - SCOPUS:85009577076
VL - 57
SP - 1121
EP - 1126
JO - Nippon Suisan Gakkaishi
JF - Nippon Suisan Gakkaishi
SN - 0021-5392
IS - 6
ER -