Analysis of Sugar Compositions of Glycoproteins Electroblotted onto PVDF Membranes

Koji Muramoto, Hisao Kamiya

    研究成果: Article査読

    5 被引用数 (Scopus)

    抄録

    A method was developed for analysis of sugar compositions of glycoproteins electroblotted onto polyvinylidene difluoride (PVDF) membranes after SDS-polyacrylamide gel electrophoresis. It was based on hydrolysis of glycoproteins on PVDF membranes and precolumn derivatization of liberated neutral sugars, sialic acids and aminosugars, respectively, and isocratic reversed-phase high-performance liquid chromatographies of these derivatives. The presence of sialoglycoproteins in ecbinoderm shell matrixes was demonstrated by this method.

    本文言語English
    ページ(範囲)1121-1126
    ページ数6
    ジャーナルNIPPON SUISAN GAKKAISHI
    57
    6
    DOI
    出版ステータスPublished - 1991 1月 1

    ASJC Scopus subject areas

    • 水圏科学

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