Analysis of short DNA fragments from Holocene peatmoss samples

Y. Suyama, U. Gunnarsson, L. Parducci

研究成果: Article査読

10 被引用数 (Scopus)

抄録

This paper describes our recent attempt to isolate and analyse DNA from old plant remains of the common peatmoss Sphagnum fuscum retrieved from a peat core collected in the mire Fuglmyra, in central Norway. DNA was recoverable and usable from subfossilized (10-450 years old) plant remains of the peat-moss. A chloroplast (trnL) and two nuclear (ITS2 and RAPDf) regions were co-amplified from 80 samples of different ages. The RAPDf region was the only variable one with three different haplotypes found among five samples. Comparison of the ancient sequences with modern sequences found in the extant population occurring at the same site ascertained a genetic link between modem and fossil samples of this species. This retrieval of ancient DNA from subfossilized moss remains isolated from peat cores has important implications for the palaeoecology of peatmosses by allowing direct estimates of plant population dynamics in space and time.

本文言語English
ページ(範囲)1003-1006
ページ数4
ジャーナルHolocene
18
6
DOI
出版ステータスPublished - 2008 9

ASJC Scopus subject areas

  • 地球変動および惑星変動
  • 考古学
  • 生態学
  • 地表過程
  • 古生物学

フィンガープリント

「Analysis of short DNA fragments from Holocene peatmoss samples」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル