TY - JOUR
T1 - Analysis of PPAR alpha function in human kidney cell line using siRNA.
AU - Tachibana, Keisuke
AU - Anzai, Naohiko
AU - Ueda, Chihiro
AU - Katayama, Tatsuya
AU - Kirino, Takayoshi
AU - Takahashi, Rika
AU - Yamasaki, Daisuke
AU - Ishimoto, Kenji
AU - Tanaka, Toshiya
AU - Hamakubo, Takao
AU - Ueda, Yukihiko
AU - Arai, Hiroyuki
AU - Sakai, Juro
AU - Kodama, Tatsuhiko
AU - Doi, Takefumi
PY - 2006
Y1 - 2006
N2 - The peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors that belong to the nuclear hormone receptor superfamily. PPARalpha is mainly expressed in the liver, kidney, heart and muscle. PPARalpha activates fatty acid catabolism, stimulates gluconeogenesis and ketone body synthesis and is involved in the control of lipoprotein assembly. Although PPARalpha is well characterized in the liver, its physiological function is unknown in the kidney. To investigate the intimate function of PPARalpha in the kidney, we analyzed the target gene expression in human metastatic renal cell carcinoma cell line, Caki-1, using small interfering RNA (siRNA) against PPARalpha and real-time RT-PCR methods. We found that some selected genes (long-chain fatty-acid-CoA ligase (FACL1), carnitine palmitoyltransferase 1A (CPT1A), adipose differentiation-related protein (ADRP) and aquaporin 3 (AQP3)) were down-regulated by PPARalpha siRNA. These results suggest that PPARalpha regulates fatty acid metabolism and body water homeostasis in this cell line.
AB - The peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors that belong to the nuclear hormone receptor superfamily. PPARalpha is mainly expressed in the liver, kidney, heart and muscle. PPARalpha activates fatty acid catabolism, stimulates gluconeogenesis and ketone body synthesis and is involved in the control of lipoprotein assembly. Although PPARalpha is well characterized in the liver, its physiological function is unknown in the kidney. To investigate the intimate function of PPARalpha in the kidney, we analyzed the target gene expression in human metastatic renal cell carcinoma cell line, Caki-1, using small interfering RNA (siRNA) against PPARalpha and real-time RT-PCR methods. We found that some selected genes (long-chain fatty-acid-CoA ligase (FACL1), carnitine palmitoyltransferase 1A (CPT1A), adipose differentiation-related protein (ADRP) and aquaporin 3 (AQP3)) were down-regulated by PPARalpha siRNA. These results suggest that PPARalpha regulates fatty acid metabolism and body water homeostasis in this cell line.
UR - http://www.scopus.com/inward/record.url?scp=36049041867&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=36049041867&partnerID=8YFLogxK
U2 - 10.1093/nass/nrl128
DO - 10.1093/nass/nrl128
M3 - Article
C2 - 17150915
AN - SCOPUS:36049041867
SP - 257
EP - 258
JO - Nucleic acids symposium series (2004)
JF - Nucleic acids symposium series (2004)
SN - 1746-8272
IS - 50
ER -