Analysis of phenylthiocarbamyl-amino acids at pico-mole level by high performance liquid chromatography and application to plant materials

Tomoyuki Yamaya, Hideaki Matsumoto, Tomoyuki Yamaya

研究成果: Article査読

13 被引用数 (Scopus)

抄録

Pre-column derivatization with DNS (BAYER et al. 1976), PTH (BLACK and COON 1982; KOBAYASHI and TARR 1986), OPA (LINDROTH and MOPPER 1979: WINSPEAR and OAKS 1983), or PTC (BIDLINGMEYER et al. 1984; KOBAYASHI and TARR 1986; KUWANO et al. 1987) enables the separation of ammo acids by reversed-phase HPLC. Analysis of amino acids with these methods was efficient and accurate. However, one of the drawbacks of the methods using OPA is the lack of derivative stability and of reactivity with several amino acids, such as proline (LINDROTH and MOPPER 1979; WINSPEAR and OAKS 1983). Interference from reagent peaks is also a major problem with DNS (BAYER et al. 1976).

本文言語English
ページ(範囲)297-302
ページ数6
ジャーナルSoil Science and Plant Nutrition
34
2
DOI
出版ステータスPublished - 1988 6月
外部発表はい

ASJC Scopus subject areas

  • 土壌科学
  • 植物科学

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