TY - JOUR
T1 - Analysis of dislocation images in X-ray topography of protein crystals
T2 - Tetragonal hen egg-white lysozyme crystals
AU - Koizumi, H.
AU - Tachibana, M.
AU - Yoshizaki, I.
AU - Kojima, K.
N1 - Funding Information:
The synchrotron radiation experiments were carried out at Photon Factory (PF) under the auspices of the PF Program Advisory Committee of the High Energy Accelerator Research Organization (KEK) (Proposal Nos. 2003G022, 2004G173 and 2005G015) and at SPring-8 with approval of the Japan Synchrotron Radiation Research Institute (Proposal Nos. 2004A0034-ND3c-np and 2005A0405-ND3c-np). M.T. and K.K. acknowledge the financial support of Yokohama City University.
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2005/11/1
Y1 - 2005/11/1
N2 - Dislocation images in X-ray topography of protein crystals such as tetragonal hen egg-white lysozyme crystals were analyzed. Not only extinction but also double contrast of dislocation images are clearly observed on the X-ray topographs. It should be noted that the observed image widths of the dislocation contrasts are much less than those calculated on the basis of the kinematical theory in X-ray topography, which has been successfully applied for inorganic crystals and organic crystals of small molecules so far. Moreover, in tetragonal HEW lysozyme crystals, the rocking curve widths of the perfect crystal related to the kinematical theory are less than the measured ones by two orders of magnitude. This discrepancy is consistent with that in the image width of the dislocation contrast. From this correlation, it is suggested that the larger rocking curve width, or higher mosaicity, is mainly responsible for the observed image width in the grown crystals.
AB - Dislocation images in X-ray topography of protein crystals such as tetragonal hen egg-white lysozyme crystals were analyzed. Not only extinction but also double contrast of dislocation images are clearly observed on the X-ray topographs. It should be noted that the observed image widths of the dislocation contrasts are much less than those calculated on the basis of the kinematical theory in X-ray topography, which has been successfully applied for inorganic crystals and organic crystals of small molecules so far. Moreover, in tetragonal HEW lysozyme crystals, the rocking curve widths of the perfect crystal related to the kinematical theory are less than the measured ones by two orders of magnitude. This discrepancy is consistent with that in the image width of the dislocation contrast. From this correlation, it is suggested that the larger rocking curve width, or higher mosaicity, is mainly responsible for the observed image width in the grown crystals.
UR - http://www.scopus.com/inward/record.url?scp=33644612265&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33644612265&partnerID=8YFLogxK
U2 - 10.1080/14786430500267752
DO - 10.1080/14786430500267752
M3 - Article
AN - SCOPUS:33644612265
VL - 85
SP - 3709
EP - 3717
JO - Philosophical Magazine
JF - Philosophical Magazine
SN - 1478-6435
IS - 31
ER -