Analysis of ciliary motion and the axonemal structure in the mouse respiratory cilia

Hironori Ueno, Takuji Ishikawa, Khanh Huy Bui, Kohsuke Gonda, Takashi Ishikawa, Takami Yamaguchi

研究成果: Conference contribution

抄録

Mucociliary clearance on the surface of the tracheal lumen is an important component of lung defense against dust mites and viruses. However, the axonemal structure that achieves effective ciliary motion and the mechanisms by which discretely distributed ciliary cells generate directional flow are unknown. In this study, we examined individual ciliary motion with 7-9-nm spatial precision by labeling the ciliary tip with quantum dots, and detected an asymmetric beating pattern. Cryo-electron tomography revealed that the densities of two inner dynein arms were missing from at least two doublet microtubules in the axonemal structure. Although the flow directions generated by individual ciliated cells were unsteady and diverse, the time- and space-averaged velocity field was found to be directional. These results indicate that the asymmetric ciliary motion is driven by the asymmetric axonemal structure, and it generates overall directional flow from the lungs to the oropharynx on sparsely distributed ciliated cells.

本文言語English
ホスト出版物のタイトルASME 2012 Summer Bioengineering Conference, SBC 2012
ページ1259-1260
ページ数2
DOI
出版ステータスPublished - 2012
イベントASME 2012 Summer Bioengineering Conference, SBC 2012 - Fajardo, Puerto Rico
継続期間: 2012 6 202012 6 23

出版物シリーズ

名前ASME 2012 Summer Bioengineering Conference, SBC 2012

Other

OtherASME 2012 Summer Bioengineering Conference, SBC 2012
国/地域Puerto Rico
CityFajardo
Period12/6/2012/6/23

ASJC Scopus subject areas

  • バイオエンジニアリング

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