In the recent tissue engineering, the necessity of the control of cellular behavior is increasing more and more. In this study, we quantified cell attachment and spreading by unit of cell by using artificial cell membrane, micro channel and QCM, and observed cellular behavior, for aiming at the clarification of the way of the control of cellular behavior and mechanism of a cell. Micro channel is made of polydimethylsiloxane (PDMS) and surface in channel is coated with 2-methacryloyloxyehyl phosphorylcholine(MPC) polymer, which was bioinspired from the cell membrane, to inhibit the non-specific cell adhesion on the surface. By using this micro channel, we succeeded in taking out a single cell. From measurement result by QCM of 50000,5000,500cells/ml L929 cell suspension, cells grew steadily on gold electrode, and the resistance change of QCM electrode are related to the number of adherent cells. From the results, it is concluded that the single cell analysis system combined by QCM and micro-channel which have the function of cell separation is constructed.
|出版ステータス||Published - 2006 10 19|
|イベント||55th SPSJ Annual Meeting - Nagoya, Japan|
継続期間: 2006 5 24 → 2006 5 26
|Other||55th SPSJ Annual Meeting|
|Period||06/5/24 → 06/5/26|
ASJC Scopus subject areas