Anaerobic treatment of N, N-dimethylformamide-containing wastewater by co-culturing two sources of inoculum

Zhe Kong, Lu Li, Rei Kurihara, Kengo Kubota, Yu You Li

研究成果: Article査読

27 被引用数 (Scopus)

抄録

The complete methanogenic degradation of N, N-dimethylformamide (DMF) was achieved in this study. Initially, DMF was found to be feebly degradable by a lab-scale submerged anaerobic membrane bioreactor (SAnMBR) using normal anaerobic digestion sludge (ADS) even after 120-day's culturing. However, aerobic DMF-degrading activated sludge (AS) was rapidly cultivated in a continuous aeration reactor (CAR). A specially designed anaerobic co-cultured sludge (ACS) made by artificially mixing AS with ADS was successfully domesticated by a long term repeated batch experiment. The results demonstrated that ACS could effectively degrade over 5000 mg L−1 DMF for methane recovery. The metabolic pathway and stoichiometric equation of DMF methanogenic degradation were also revealed and verified in detail. It was confirmed that under the anaerobic condition, with the help of enzyme, DMF converts into dimethylamine and formic acid, and the intermediates are effectively fermented through methylotrophic/hydrogenotrophic methanogenesis. Analysis of the microbial community suggested that some facultatively anaerobic bacteria played the key roles in methanogenic degradation due to their DMF-hydrolyzing ability. By co-culturing two sources of inoculum under the anaerobic condition, the symbiosis of facultatively anaerobic DMF-hydrolyzing bacteria and methylotrophic/hydrogenotrophic methanogens makes methanogenic degradation of DMF available. This study also provides a novel sludge cultivation method for anaerobic treatment of degradation-resistant organics.

本文言語English
ページ(範囲)228-239
ページ数12
ジャーナルWater Research
139
DOI
出版ステータスPublished - 2018 8 1

ASJC Scopus subject areas

  • 生態モデリング
  • 水の科学と技術
  • 廃棄物管理と処理
  • 汚染

フィンガープリント

「Anaerobic treatment of N, N-dimethylformamide-containing wastewater by co-culturing two sources of inoculum」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル