TY - JOUR
T1 - Anaerobic reduction of nitrate to nitrous oxide is lower in Bradyrhizobium japonicum than in Bradyrhizobium diazoefficiens
AU - Siqueira, Arthur Fernandes
AU - Minamisawa, Kiwamu
AU - Sánchez, Cristina
N1 - Funding Information:
This work was supported by a Grant-in-Aid for Scientific Research (A) 26252065 from the Ministry of Education, Culture, Sports, Science and Technology of Japan, and by a grant from the Science and Technology Research Promotion Program for Agriculture, Forestry, Fisheries and Food Industry. A.F.S. was supported by fellowships from the National Council for Scientific and Technological Development-Brazil and the Japan Society for the Promotion of Science.
Publisher Copyright:
© 2017, Japanese Society of Microbial Ecology. All rights reserved.
PY - 2017
Y1 - 2017
N2 - When soil oxygen levels decrease, some bradyrhizobia use denitrification as an alternative form of respiration. Bradyrhizobium diazoefficiens (nos+) completely denitrifies nitrate (NO3 -) to dinitrogen, whereas B. japonicum (nos-) is unable to reduce nitrous oxide to dinitrogen. We found that anaerobic growth with NO3 - as the electron acceptor was significantly lower in B. japonicum than in B. diazoefficiens, and this was not explained by the absence of nos in B. japonicum. Our results indicate that the reason for the limited growth of B. japonicum is weak NO3 - reduction due to impaired periplasmic nitrate reductase activity, which may rely on posttranscriptional events.
AB - When soil oxygen levels decrease, some bradyrhizobia use denitrification as an alternative form of respiration. Bradyrhizobium diazoefficiens (nos+) completely denitrifies nitrate (NO3 -) to dinitrogen, whereas B. japonicum (nos-) is unable to reduce nitrous oxide to dinitrogen. We found that anaerobic growth with NO3 - as the electron acceptor was significantly lower in B. japonicum than in B. diazoefficiens, and this was not explained by the absence of nos in B. japonicum. Our results indicate that the reason for the limited growth of B. japonicum is weak NO3 - reduction due to impaired periplasmic nitrate reductase activity, which may rely on posttranscriptional events.
KW - Bacterial physiology
KW - Bradyrhizobium
KW - Denitrification
KW - Nitrogen cycle
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U2 - 10.1264/jsme2.ME17081
DO - 10.1264/jsme2.ME17081
M3 - Article
C2 - 29109361
AN - SCOPUS:85040114148
VL - 32
SP - 398
EP - 401
JO - Microbes and Environments
JF - Microbes and Environments
SN - 1342-6311
IS - 4
ER -