TY - JOUR
T1 - Anaerobic nitrogen-fixing consortia consisting of clostridia isolated from gramineous plants
AU - Minamisawa, Kiwamu
AU - Nishioka, Kiyo
AU - Miyaki, Taro
AU - Ye, Bin
AU - Miyamoto, Takuya
AU - You, Mu
AU - Saito, Asami
AU - Saito, Masanori
AU - Barraquio, Wilfredo L.
AU - Teaumroong, Neung
AU - Sein, Than
AU - Sato, Tadashi
PY - 2004/5
Y1 - 2004/5
N2 - We report here the existence of anaerobic nitrogen-fixing consortia (ANFICOs) consisting of N2-fixing clostridia and diverse nondiazotrophic bacteria in nonleguminous plants; we found these ANFICOs while attempting to overcome a problem with culturing nitrogen-fixing microbes from various gramineous plants. A major feature of ANFICOs is that N2 fixation by the anaerobic clostridia is supported by the elimination of oxygen by the accompanying bacteria in the culture. In a few ANFICOs, nondiazotrophic bacteria specifically induced nitrogen fixation of the clostridia in culture. ANFICOs are widespread in wild rice species and pioneer plants, which are able to grow in unfavorable locations. These results indicate that clostridia are naturally occurring endophytes in gramineous plants and that clostridial N 2 fixation arises in association with nondiazotrophic endophytes.
AB - We report here the existence of anaerobic nitrogen-fixing consortia (ANFICOs) consisting of N2-fixing clostridia and diverse nondiazotrophic bacteria in nonleguminous plants; we found these ANFICOs while attempting to overcome a problem with culturing nitrogen-fixing microbes from various gramineous plants. A major feature of ANFICOs is that N2 fixation by the anaerobic clostridia is supported by the elimination of oxygen by the accompanying bacteria in the culture. In a few ANFICOs, nondiazotrophic bacteria specifically induced nitrogen fixation of the clostridia in culture. ANFICOs are widespread in wild rice species and pioneer plants, which are able to grow in unfavorable locations. These results indicate that clostridia are naturally occurring endophytes in gramineous plants and that clostridial N 2 fixation arises in association with nondiazotrophic endophytes.
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U2 - 10.1128/AEM.70.5.3096-3102.2004
DO - 10.1128/AEM.70.5.3096-3102.2004
M3 - Article
C2 - 15128572
AN - SCOPUS:2442658186
SN - 0099-2240
VL - 70
SP - 3096
EP - 3102
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
IS - 5
ER -