An oxygen ultramicroelectrode was fabricated by pyrolysis of butane inside a glass capillary, followed by electrodeposition of Pt. The intracellular determination of oxygen in an algal protoplast (diameter, approx. 80 μm) with the ultramicroelectrode indicated that at a light intensity of 25 klx, the oxygen efflux from a single protoplast caused by the light reactions is approx. 5·10-13 mol/s and that corresponding to the dark reactions approx, 1 · 10-12 mol/s.
|ジャーナル||Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular|
|出版ステータス||Published - 1992 7 6|
ASJC Scopus subject areas
- Structural Biology
- Molecular Biology