An ultramicroelectrode for determination of intracellular oxygen. Light-irradiation-induced change in oxygen concentration in an algal protoplast

Tomokazu Matsue, Shinji Koike, Takayuki Abe, Takeyuki Itabashi, Isamu Uchida

研究成果: Article査読

34 被引用数 (Scopus)

抄録

An oxygen ultramicroelectrode was fabricated by pyrolysis of butane inside a glass capillary, followed by electrodeposition of Pt. The intracellular determination of oxygen in an algal protoplast (diameter, approx. 80 μm) with the ultramicroelectrode indicated that at a light intensity of 25 klx, the oxygen efflux from a single protoplast caused by the light reactions is approx. 5·10-13 mol/s and that corresponding to the dark reactions approx, 1 · 10-12 mol/s.

本文言語English
ページ(範囲)69-72
ページ数4
ジャーナルBiochimica et Biophysica Acta (BBA)/Protein Structure and Molecular
1101
1
DOI
出版ステータスPublished - 1992 7 6

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology

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