An LC/ESI-SRM/MS method to screen chemically modified hemoglobin: simultaneous analysis for oxidized, nitrated, lipidated, and glycated sites

研究成果: Article査読

4 被引用数 (Scopus)

抄録

Proteins are continuously exposed to various reactive chemical species (reactive oxygen/nitrogen species, endogenous/exogenous aldehydes/epoxides, etc.) due to physiological and chemical stresses, resulting in various chemical modifications such as oxidation, nitration, glycation/glycoxidation, lipidation/lipoxidation, and adduct formation with drugs/chemicals. Abundant proteins with a long half-life, such as hemoglobin (Hb, t1/2 63 days, ∼150 mg/mL), are believed to be major targets of reactive chemical species that reflect biological events. Chemical modifications on Hb have been investigated mainly by mechanistic in vitro experiments or in vivo/clinical experiments focused on single target modifications. Here, we describe an optimized LC/ESI-SRM/MS method to screen oxidized, nitrated, lipidated, and glycated sites on Hb. In vivo preliminary results suggest that this method can detect simultaneously the presence of oxidation (+16 Da) of α-Met32, α-Met76, β-Met55, and β-Trp15 and adducts of malondialdehyde (+54 Da) and glycation (+162 Da) of β-Val1 in a blood sample from a healthy volunteer. [Figure not available: see fulltext.]

本文言語English
ページ(範囲)5379-5392
ページ数14
ジャーナルAnalytical and Bioanalytical Chemistry
408
19
DOI
出版ステータスPublished - 2016 7 1

ASJC Scopus subject areas

  • 分析化学
  • 生化学

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