An interface chip connection between capillary electrophoresis and thermal lens microscope

Kenji Uchiyama; Akihide Hibara; Kiichi Sato; Hideaki Hisamoto; Manabu Tokeshi; Takehiko Kitamori

doi:10.1002/elps.200390011

An interface chip connection between capillary electrophoresis and thermal lens microscope

Kenji Uchiyama, Akihide Hibara, Kiichi Sato, Hideaki Hisamoto, Manabu Tokeshi, Takehiko Kitamori

研究成果: Review article › 査読

37 被引用数 (Scopus)

抄録

A thermal lens microscope (TLM) detection of capillary electrophoresis (CE) utilizing microchip technology was developed. Fused-silica capillaries with an inner diameter of 50 μm were directly connected to a microchannel in a microchip. The detection limit by TLM was estimated as 2.8 × 10^-7 absorbance by measuring pure water. The detection limit of derivatized amino acids determined by CE-TLM was estimated as 2.4 × 10^-8 M, which was 100 times lower than that of conventional absorbance detection.

本文言語	English
ページ（範囲）	179-184
ページ数	6
ジャーナル	Electrophoresis
巻	24
号	1-2
DOI	https://doi.org/10.1002/elps.200390011
出版ステータス	Published - 2003 1月
外部発表	はい

ASJC Scopus subject areas

分析化学
生化学
臨床生化学

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10.1002/elps.200390011

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title = "An interface chip connection between capillary electrophoresis and thermal lens microscope",

abstract = "A thermal lens microscope (TLM) detection of capillary electrophoresis (CE) utilizing microchip technology was developed. Fused-silica capillaries with an inner diameter of 50 μm were directly connected to a microchannel in a microchip. The detection limit by TLM was estimated as 2.8 × 10-7 absorbance by measuring pure water. The detection limit of derivatized amino acids determined by CE-TLM was estimated as 2.4 × 10-8 M, which was 100 times lower than that of conventional absorbance detection.",

keywords = "Capillary electrophoresis, Dead volume-free connection, Microchip, Microfabrication, Thermal lens microscope",

author = "Kenji Uchiyama and Akihide Hibara and Kiichi Sato and Hideaki Hisamoto and Manabu Tokeshi and Takehiko Kitamori",

year = "2003",

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T1 - An interface chip connection between capillary electrophoresis and thermal lens microscope

AU - Uchiyama, Kenji

AU - Hibara, Akihide

AU - Sato, Kiichi

AU - Hisamoto, Hideaki

AU - Tokeshi, Manabu

AU - Kitamori, Takehiko

PY - 2003/1

Y1 - 2003/1

N2 - A thermal lens microscope (TLM) detection of capillary electrophoresis (CE) utilizing microchip technology was developed. Fused-silica capillaries with an inner diameter of 50 μm were directly connected to a microchannel in a microchip. The detection limit by TLM was estimated as 2.8 × 10-7 absorbance by measuring pure water. The detection limit of derivatized amino acids determined by CE-TLM was estimated as 2.4 × 10-8 M, which was 100 times lower than that of conventional absorbance detection.

AB - A thermal lens microscope (TLM) detection of capillary electrophoresis (CE) utilizing microchip technology was developed. Fused-silica capillaries with an inner diameter of 50 μm were directly connected to a microchannel in a microchip. The detection limit by TLM was estimated as 2.8 × 10-7 absorbance by measuring pure water. The detection limit of derivatized amino acids determined by CE-TLM was estimated as 2.4 × 10-8 M, which was 100 times lower than that of conventional absorbance detection.

KW - Capillary electrophoresis

KW - Dead volume-free connection

KW - Microchip

KW - Microfabrication

KW - Thermal lens microscope

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DO - 10.1002/elps.200390011

M3 - Review article

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AN - SCOPUS:0037269140

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SP - 179

EP - 184

JO - Electrophoresis

JF - Electrophoresis

SN - 0173-0835

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An interface chip connection between capillary electrophoresis and thermal lens microscope

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