An immunochemical study of δ-aminolevulinate synthase and δ-aminolevulinate dehydratase in liver and erythroid cells of rat

Masayuki Yamamoto, Hiroyoshi Fujita, Norimichi Watanabe, Norio Hayashi, Goro Kikuchi

研究成果: Article査読

23 被引用数 (Scopus)

抄録

The relationship between erythroid δ-aminolevulinate (ALA) synthase and hepatic ALA synthase in rat was analyzed immunochemically, using antibodies directed against rat liver ALA synthase and against chicken liver ALA synthase. Rat erythroid ALA synthase showed no cross-reactivity with anti-liver ALA synthase antibodies, but hepatic ALA synthases from rat, mouse, and chicken share substantial cross-reactivity with one another. These results clearly distinguish the isozyme relationship between erythroid ALA synthase and hepatic ALA synthase in rat and suggest that there may be at least two different ALA synthase genes in rat. ALA dehydratase in rat liver, on the other hand, could not be immunochemically distinguished from ALA dehydratase in rat erythroid cells when antibody against rat erythroid ALA dehydratase was used. The finding that erythroid ALA synthase is an entity different from hepatic ALA synthase may provide a clue to understanding the different features in hepatic and erythropoietic porphyrias.

本文言語English
ページ(範囲)76-83
ページ数8
ジャーナルArchives of Biochemistry and Biophysics
245
1
DOI
出版ステータスPublished - 1986 2 15

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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