An assay for α 1,6-Fucosyltransferase (FUT8) activity based on the HPLC separation of a reaction product with fluorescence detection

Hideyuki Ihara, Hiroki Tsukamoto, Naoyuki Taniguchi, Yoshitaka Ikeda

研究成果: Chapter

4 被引用数 (Scopus)

抄録

N-Glycans with an α-fucose unit linked to the 6-position of the innermost GlcNAc are widely distributed among the animal kingdom, from worms and insects to human. This α1,6-linked fucosyl residue, frequently referred to as a core fucose, is formed via the action of an α1,6- fucosyltransferase, the mammalian ortholog which is systematically called FUT8. In mammals, it is well known that the extent of core-fucosylation in cellular and secreted glycoproteins varies, e.g., according to differentiation and carcinogenesis of the cells. This chapter describes a method for the sensitive and quantitative assay of FUT8 activity using a fluorescence-labeled oligosaccharyl asparagine derivative as the glycosyl acceptor substrate.

本文言語English
ホスト出版物のタイトルGlycosyltransferases
ホスト出版物のサブタイトルMethods and Protocols
出版社Humana Press Inc.
ページ335-348
ページ数14
ISBN(印刷版)9781627034647
DOI
出版ステータスPublished - 2013

出版物シリーズ

名前Methods in Molecular Biology
1022
ISSN(印刷版)1064-3745

ASJC Scopus subject areas

  • 分子生物学
  • 遺伝学

フィンガープリント

「An assay for α 1,6-Fucosyltransferase (FUT8) activity based on the HPLC separation of a reaction product with fluorescence detection」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル