Amperometric detection of the bacterial metabolic regulation with a microbial array chip

Kuniaki Nagamine, Nobuto Matsui, Takatoshi Kaya, Tomoyuki Yasukawa, Hitoshi Shiku, Toru Nakayama, Tokuzo Nishino, Tomokazu Matsue

研究成果: Article査読

18 被引用数 (Scopus)

抄録

A microbial array chip with collagen gel spots entrapping living bacterial cells has been applied to investigate the metabolic regulation in Paracoccus denitrificans. Scanning electrochemical microscopy (SECM) was used to monitor the ferrocyanide production that reflects the electron flow in the respiratory chain located within the internal membrane of P. denitrificans. The ferrocyanide production from P. denitrificans largely depends on the types of the carbon source (glucose or lactate), suggesting that the electron flow rate in the respiratory chain depends on the activity of the metabolic pathway located up-stream of the respiratory chain. More importantly, it was found that the enzymes affecting glucose catabolic reactions were significantly up-regulated in cultures with a nutrient agar medium containing d-(+)-glucose as a sole carbon source. Enzyme assays using crude extracts of P. denitrificans were carried out to identify the enzymes expressed at a higher level in cultures supplemented with d-(+)-glucose. It was confirmed that the pyruvate kinase and enzymes of the overall Entner-Doudoroff pathway were highly induced in cultures containing d-(+)-glucose.

本文言語English
ページ(範囲)145-151
ページ数7
ジャーナルBiosensors and Bioelectronics
21
1
DOI
出版ステータスPublished - 2005 7 15

ASJC Scopus subject areas

  • バイオテクノロジー
  • 生物理学
  • 生体医工学
  • 電気化学

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